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1.3.2 Post-Process Platelet Yields

Table II provides summary data for platelet recovery in processed samples.

Table II: Post –Process Platelet Yields

Mean ą 1SD (Range)

PRP volume 4 ml

PRP @ 0hr

PRP @ 4hr

Yield (%)

68 ą17 (48-106)

65 ą 16 (44-96)

1.3.3 Platelet concentration (times baseline) in time 0 and Time +4 hr CPP

Table IV lists the calculated increase in platelet concentration in CPP samples above baseline in both Time 0 and 4 hr post-processing samples.

Table III: Post –Process Platelet Concentration

Mean ą 1SD (Range)

PRP volume 4 ml

PRP @ 0hr platelet

(Times Baseline)

PRP @ 4hr

(Times Baseline)

Correlation Coefficient

10 ą 3 (5.8-14)

9 ą 3 (6.6-14)

Post –Process Bone Marrow Aspirate
Mean ą 1SD (Range)
Bone Marrow Concentrate (BMC) volume 4 ml

Volume

Total Nuclear

Cells x 103/ľL

Concentration

Above Baseline

Bone Marrow Aspirate Baseline

16-23

1.0X

Bone Marrow Concentrate

170-271

11.5X

Table I: Cell concentrations in 60 ml bone marrow aspirate and in 4ml bone marrow concentrate sample: Total nucleated cells (TNC), Platelets (Plt) and CD34+ hematopoietic stem cells (HSC), n=4

Bone Marrow Aspirate Baseline			Bone Marrow Concentrate
TNC x 10⁶	HSC x 10⁶	Plt x 10⁶	TNC x 10⁶	HSC x 10⁶	Plt x 10⁶
1161 ą 239	9.0 ą 1.3	10,830 ą 1836	894 ą 232	6.9 ą 1.4	7,623 ą 1432
Concentration factor (times baseline) In 4ml buffy coat BMC			11.6	11.5	10.6

The recovery of TNC, Plt and CD34+ HCS were calculated and are shown in Figure 1.

Figure 1. Recovery of TNC, Plt and CD34+ HCS

	Aspirate Volume (mL)	TNC In BAC x106	TNC x106 Per 60mL Aspirate
GenesisCS	60	896	896
Harvest BMAC	60	826	826
Gambro 2991	300	1170	358
Ficoll	60	373	373

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